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Smart Mobile Technology

Now Smartphones Can Be Turned Into Fluorescence Microscopes

smartmobtech by smartmobtech
October 16, 2019
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Scientists in the U.S. and China have established a method to change a smartphone into a fluorescence microscope. The miniature smartphone-fluorescence microscope (HSFM) device permits complicated biomedical evaluations both swiftly and inexpensively. Traditional fluorescence microscopes play a significant part to spot different cells and proteins, but they are Weighty and problematic for point-of-care diagnoses.

Currently, writing in Light: Science & Applications, Bo Dai, and a collaborative research team detailed the use of liquid polymers to create small two-droplet lenses tinted with colored solvents. The lenses were harmonious across several different smartphone cameras. The low-cost, trial setup allowed them to see and count cells, observe the expression of fluorescently tagged genes, and differentiate between tumors and normal tissues. The easily available and inexpensive smartphone technology can contribute to cost-effective science and will lead to better management of onsite and economically feasible personalized medicine.

Fluorescence microscopy is universal in multiple disciplines, including the healthcare industry, cell and molecular biology, environmental monitoring and food sanitation. In clinical applications and cell and molecular biology, fluorescent imaging can identify and track proteins, cells and other molecules of interest with high receptiveness and accuracy. Traditional fluorescence microscopes are typically made with large components, which make them extremely difficult for point-of-care analysis in resource-limited regions. Thus, handy microscopes are a vital development on a perfect smartphone platform for flexibility and availability to a range of users.

Fluorescence Microscope and its uses
(a) A smartphone fluorescence microscope can be used as digital tool for cancer mutation detection. (b) The smartphone image of in situ RCA assays in fixed model cell lines superimposes 2 fluorescent color channels onto a bright-field channel of the mobile microscope. (c) The same smartphone microscope can image in situ KRAS mutation in colon cancer tumor sections.

Researchers had earlier used smartphone-based microscopes to image waterborne parasites,  human blood cells, and human cytomegalovirus. For these research labors they included important elements such as external lenses for optical imaging, light-emitting diodes (LEDs) for illumination, and magnification as well as fluorescence emission filtering to route light. Polymer lenses are easy to build and deliver high-resolving power to construct a ‘do-it-yourself’ microscope for resource-limited applications. However, due to the varied models of smartphones currently available, researchers wish to make an attachment for smartphone-based microscopy whose structure is autonomous from a particular phone model.

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To counter this challenge in the present work, Dai et al. built a low-cost handheld smartphone fluorescence microscope (HFSM) in a manageable size. The HRSM incorporated a single compact and multifunctional color lens to change any smartphone version into a fluorescence microscope without altering the attachment design between phones. The experimental design lessened the HRFM device complexity and permitted its acceptance across a variety of smartphones.

 

Tags: fluorescence microscopemicroscopesmartphone conversion
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